Figure 5.

Attenuated association of APP with X11L in the brains of Alcα-deficient mice. A, co-immunoprecipitation of APP with X11L in the presence or absence of Alcα. Crude membrane fractions (500 µg of protein) of the hippocampus and cerebral cortex of WT (+/+) and Alcα-deficient (−/−) mice were subject to immunoprecipitation with anti-X11L antibody, and the immunoprecipitates were analyzed by immunoblotting with anti-APP, anti-X11L, and anti-Alcα antibodies. B, the band densities of APP in A were quantified and standardized against X11L in the immunocomplex. APPs, mature APP plus immature APP; imAPP, immature APP; mAPP, mature APP. WT was assigned a reference value of 1.0. Statistical significance was analyzed using three independent experiments (n = 3 mice/group; unpaired t test; *, p < 0.05; **, p < 0.01). Data represent means ± S.E. (error bars).