Figure 2.

(a) Single cell and cfDNA genomic preparation for next-generation sequencing and CNA analysis. Single cells are extracted from slides with a robotic micromanipulator prior to whole genome amplification and DNA purification followed by Illumina DNA library preparation for sequencing. CfDNA is extracted from plasma and Illumina DNA libraries are constructed similarly to single cells. CNA profiles are created using the human genome as reference where copy number is calculated then displayed as the ratio to the median. (b) Targeted proteomic analysis via IMC. Slides previously labeled with fluorescent antibodies are stained with 21 metal-conjugated antibodies. Regions of interest are laser ablated with plasma ionization and ions are detection using Cytometry by Time of Flight (CyTOF) technology. Rasterized images are generated from ion count data, and protein expression on tumor cells are scored for expression levels.