| NaCl (31434, Riedel) |
Dissecting/stereo microscope (EZ4 HD, Leica) |
Petri dish 90 mm (101RT/C, Thermo Scientific) |
| KCl (31248, Riedel) |
CO2 Incubator (Heracell, Heraeus) |
Petri dish 60 mm (628102, Greiner) |
| Na2HPO4 × 2H2O (0326, J.T. BAKER) |
Centrifuge 5810 (Eppendorf) |
Petri dish 35 mm (627102, Greiner) |
| KH2PO4 (4871, Merck) |
Scissors (RU 1003-14 Rudolf) |
Multidish four-well plate (176740, Thermo scientific) |
| HBBS (14170088, Gibco) |
Spring scissors (15004-08 Fine Science Tools) |
Superfrost slides (J1800AMNT, Thermo Scientific) |
| 100 mM sodium pyruvate (11360039, Gibco) |
Forceps (11000-13 Fine Science Tools) |
Coverslips (Round 13 mm, Thermo Scientific) |
| 1 M HEPES pH 7,2 (101926, ICN) |
Curved forceps (11271-30/Dumont#7 Fine Science Tools) |
C-Chip Disposable Hemocytometer (Bürker, LabTech) |
| DMEM (BE12-614F, BioWhittaker Lonza) |
Straight forceps (11295-10/Dumont#5 Fine Science Tools) |
Microscope slides (ECN 631-1551, VWR) |
| Papain (P-4762, Sigma) |
15- and 50-ml Falcon tubes (CLS430791 Sigma) |
|
| DNase I (D4527, Sigma) |
|
|
|
dl-Cystein HCl (C-9768, Sigma) |
|
|
| BSA (A7638, Sigma) |
|
|
|
d-(+)-Glucose anhydrous (49139, Fluka) |
|
|
| PFA (1157, J.T. BAKER) |
|
|
| Poly-l-lysine (P4707, Sigma) |
|
|
| Normal goat serum (16210-064, Lifetechnologies, Gibco) |
|
|
| Triton X-100 (93426, Fluka) |
|
|
| Anti-GFAP antibody (12389, Cell Signaling Technology) |
|
|
| Anti-NeuN antibody (MAB377X, EMD Millipore) |
|
|
| Anti-Map2 antibody (ab5392, Abcam) |
|
|
| Goat anti-rabbit 647 (A21245, Life Technologies) |
|
|
| Goat anti-mouse 568 (A11004, Life Technologies) |
|
|
| Goat anti-chicken 488 (A11039, LifeTecnologies) |
|
|
| Mounting media with DAPI (ab104139, Abcam) |
|
|
| Trypan Blue (T8154, Sigma) |
|
|
