Figure 1.

Technologies to eliminate light chain mispairing problem. CrossMab technology is based on the domain crossover in the Fab region of one arm of the bsAb. The left panel shows the CrossMab^CH1-CL format, in which CH1 domain and CL domain are exchanged on the right arm of bsAb. In the engineered CH1:CL interface technology (middle panel), several mutations were introduced to the CH1 and CL domains on the right arm, making them repulsive towards a wildtype antibody chain but suitable for pairing with each other. In a B-body construct, one arm of the bispecific IgG is a wildtype Fab, and the other Fab arm has its CH1/CL domains substituted with pairing domains derived from other human antibody domains.