Table 2.
Mechanical parameters at pCa 4.5 from myofibrils isolated from ARVMs expressing WT, K132Q, or K132R cTnI.
| RT (mN/mm2) | Po (mN/mm2) | kACT (s−1) | kTR (s−1) | t REL, LINEAR (ms) | k REL, LINEAR (s−1) | kREL, EXP (s−1) | SL (μm) | |
|---|---|---|---|---|---|---|---|---|
| cTnI WT | 3.80 ± 0.4 (n = 27) | 69.1 ± 5.6 (n = 27) | 3.38 ± 0.2 (n = 27) | 3.36 ± 0.2 (n = 27) | 51.1 ± 1.7 (n = 27) | 1.07 ± 0.1 (n = 27) | 55.6 ± 5.2 (n = 27) | 2.21 ± 0.02 (n = 27) |
| cTnI K132Q | 4.29 ± 0.4 (n = 25) | 53.6 ± 4.5 (n = 25) | 3.32 ± 0.2 (n = 25) | 3.28 ± 0.2 (n = 25) | 42.0 ± 1.4** (n = 25) | 2.03 ± 0.2**(n = 24) | 43.5 ± 3.2 (n = 25) | 2.19 ± 0.02 (n = 25) |
| cTnI K132R | 4.44 ± 0.4 (n = 24) | 68.9 ± 4.8 (n = 24) | 3.12 ± 0.2 (n = 24) | 3.36 ± 0.2 (n = 24) | 47.6 ± 1.5*(n = 24) | 1.40 ± 0.1 (n = 20) | 49.5 ± 3.5 (n = 24) | 2.21 ± 0.02 (n = 24) |
Data is expressed as mean ± standard error. N = 4 per experiment, n = total number myofibrils measured between 4 different experiments.
*
p < .05 K132Q v K132R.
**
p < .01 WT v K132Q by One-Way ANOVA with Sidak’s multiple comparison test.