Fig. 6.

THS exposure severely impairs basal transcription in NER defective cells. (A) Dermal fibroblasts from an XPA patient (XP12BE) were exposed to 5% or 2.5% THS for 24 h followed by EU incorporation and imaging as in Fig. 5. 40x magnification images are shown. (B) Quantification using ImageJ of the average fluorescence intensity of 10 cells from mock treated or THS-exposed XP12BE samples. Data represent the mean of ± SD for N=3. (C) Absence of XPA protein detectable with anti-XPA antibody in the XP-A patient cell line XP12BE is shown. The human fibroblast strain HCA2 was a normal control. XPB protein was used as a loading control.