Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Monodelphis domestica) | short-tailed opossum | Anthwal et al., 2017. DOI:10.1038/s41559-017-0093; Urban et al., 2017. DOI:10.1098/rspb.2016.2416 | ||
| Strain, strain background (Cavia porcellus) | Guinea pig | Anthwal et al., 2015. DOI:10.1186/s13227-015-0030-6 | ||
| Strain, strain background (Paroedura picta) | ocelot gecko | Zahradnicek et al., 2012. DOI:10.1111/j.1469-7580.2012.01531.x | ||
| Strain, strain background (Mus musculus) | CD1 | King’s College London | ||
| Strain, strain background (Tachyglossus aculeatus) | short-beaked echidna | University of Melbourne | ||
| Genetic reagent (Mus musculus) |
Sox9CreERT2:tdTomato | Other | RRID:MGI:4947114 | Prof Robin Lovell-Badge, Francis Crick Institute |
| Genetic reagent (Mus musculus) |
Mesp1Cre; mTmG | RIKEN | Mesp1tm2(cre)Ysa:: Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo; RRID:MGI:3702469 | |
| Biological sample (Pterobnotus quadridens) | sooty mustached bat µCT scan | Other | Karen Sears, UCLA | |
| Biological sample (Ornithorhynchus anatinus) | platypus histological slides | Hill Collection, Museum für Naturkunde, Leibniz Institute for Research on Evolution and Biodiversity, Berlin; Cambridge University Museum of Zoology; Green, 1937; Presley and Steel, 1978; Watson, 1916 | Specimen W; M45; Specimen Delta; M038; HP; Specimen Beta; HX | Museum Samples |
| Biological sample (Tachyglossus aculeatus) | short-beaked echidna histological slides | Cambridge University Museum of Zoology; Green, 1937; Presley and Steel, 1978; Watson, 1916 | HX; Echidna H.SP EC5; Echidna H.SP EC4 | Museum Samples |
| Antibody | rabbit polyclonal anti Sox9 | Millipore | ab5535; RRID:AB_2239761 | IF 1/200 |
| Antibody | chicken polyclonal anti GFP | Abcam | ab13970; RRID:AB_300798 | IF 1/500 |
| Antibody | Rat monoclonal anti RFP | Chromotek | 5f8-100; RRID:AB_2336064 | IF 1/200 |
| Antibody | Rabbit polyclonal anti Beta-catenin | Santa Cruz | sc-7199; RRID:AB_634603 | IF 1/200 |
| Antibody | mouse monoclonal anti Tenascin C | DSHB | M1B4; RRID:AB_528488 | IF 1/40 |
| Antibody | mouse monoclonal anti type 2 collagen | DSHB | II-II6B3; RRID:AB_528165 | IF 1/50 |
| Antibody | mouse monoclonal anti CD44 | DSHB | HERMES-1; RRID:AB_528148 | IHC 1/50 |
| Antibody | mouse monoclonal anti Versican | DSHB | 12C5; RRID:AB_528503 | IHC 1/50 |
| Antibody | rabbit polyclonal anti Versican V1 (DPEAAE) | Abcam | ab19345; RRID:AB_444865 | IF 1/400 |
| Antibody | Donkey Polyclonal Alexa568 conjugated anti-Rabbit | Invitrogen | A10042; RRID:AB_2534017 | IF 1/300 |
| Antibody | Donkey Polyclonal Alexa 488 conjugated anti-Rabbit | Invitrogen | A-21206; RRID:AB_2535792 | IF 1/300 |
| Antibody | Donkey Polyclonal Alexa568 conjugated anti-Mouse | Invitrogen | A10037; RRID:AB_2534013 | IF 1/300 |
| Antibody | Goat Polyclonal Alexa488 conjugated anti-Chicken | Invitrogen | A-11039; RRID:AB_2534096 | IF 1/300 |
| Antibody | Goat Polyclonal Alexa568 conjugated anti-Rat | Invitrogen | A-11077; RRID:AB_2534121 | IF 1/300 |
| Antibody | Goat Polyclonal Biotin Anti-Mouse | Dako | E0433; RRID:AB_2687905 | IHC 1/400 |
| Recombinant DNA reagent | Mouse Gdf5 in situ hybridisation probe | Tucker et al., 2004. DOI:10.1242/dev.01017 | ||
| Recombinant DNA reagent | Mouse Bapx1 in situ hybridisation probe | Tucker et al., 2004. DOI:10.1242/dev.01017 | ||
| Commercial assay or kit | ABC-HRP streptavidin kit | Vector Labs | PK-6100; RRID:AB_2336819 | |
| Commercial assay or kit | ImmPACT DAB Peroxidase Substrate | Vector Labs | SK-4105; RRID:AB_2336520 | |
| Software, algorithm | FIJI | Schindelin et al., 2015; Schindelin et al., 2012 | RRID:SCR_002285 | |
| Software, algorithm | Prism 8 | Graphpad | RRID:SCR_002798 | |
| Other | Aqueous mounting medium with DAPI | Abcam | ab104139 | |
| Other | Picro‐sirius red, haematoxylin and alcian blue trichrome staining | CCRB Histology Core at King’s College London |