Figure 10.

The decreased phosphorylation level of ribosomal protein S6 and the decreased body size of PCs were rescued by 3BDO in the cKO-PV mice. A–D′′, Coimmunostaining for pS6 (A, B, C, D) and GAD67 (A′, B′, C′, D′) at P14 (A–B′′) and P21 (C–D′′). E–H, Coimmunostaining for pS6 (E, F, G, H) and GAD67 (E′, F′, G′, H′) at P30. Arrows indicate the PCs, and arrowheads indicate the Bergmann glia in the cerebellum. I–L, Immunostaining for calbindin at P30. M, Area of PC bodies in mice at P30. The data are the mean ± SEM: 323.70 ± 3.57 μm² for vehicle-treated Ctrl mice (n = 149 PCs from 3 mice); 333.40 ± 8.24 μm² for 3BDO-treated Ctrl mice (n = 159 PCs from 3 mice); 213.04 ± 4.68 μm² for vehicle-treated cKO-PV mice (n = 85 PCs from 2 mice); and 254.00 ± 4.45 μm² for 3BDO-treated cKO-PV mice (n = 144 PCs from 3 mice). Vehicle/Ctrl mice versus 3BDO/Ctrl mice, no significance; vehicle/Ctrl mice versus vehicle/cKO-PV mice, ***p < 0.0001; vehicle/cKO-PV mice versus 3BDO/cKO-PV mice, #p = 0.0137. N, In the low rotational speed (15 and 20 rpm), 3BDO treatment rescued the motor coordination in the cKO-PV mice. p Values of 3BDO treatment of cKO-PV mice compared with vehicle treatment of Ctrl mice: 10 rpm, p > 0.999; 15 rpm, p = 0.1228; 20 rpm, p = 0.2065; 25 rpm, p = 0.0047; 30 and 33 rpm, p < 0.0001. The number of the mice used in the experiments were n = 12 for the vehicle-treated Ctrl mice group and n = 7 for the 3BDO-treated cKO-PV mice group. The data are the mean ± SEM. A two-way ANOVA; Bonferroni's post hoc analysis was used to analyze data in M and N. ***p < 0.001, **p < 0.01, ns, p ≥ 0.05. Scale bars: A–H, 100 μm; I–L, 50 μm.