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. 2020 Jun 19;7(3):ENEURO.0024-20.2020. doi: 10.1523/ENEURO.0024-20.2020

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Copyright © 2020 Arias-Cavieres et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 2. — IH suppresses NMDAr-dependent synaptic potentiation in wild-type hippocampal slices, but NMDAr-dependent LTP is unaffected by IH in the hippocampal slices from HIF1a^+/−. A, LTP was evoked using HFS in control (blue, n = 6) is attenuated by AP5 (green, n = 5). LTP_HFS is attenuated in following IH (IH₁₀, red, n = 6) and is no longer sensitive to AP5 (IH+AP5, gold, n = 5). A comparison of LTP_HFS magnitude (60 min following HFS) was performed to compare experimental conditions to control; **p < 0.01. B, LTP_TBS is readily evoked in control (light blue, n = 5) and is completely blocked by AP5 (light green, n = 5). Following IH, LTP_TBS is present (IH₁₀, pink, n = 5). Following a one-way ANOVA, a post hoc comparison of LTP_TBS magnitude (60 min following TBS) was performed to compare experimental conditions to control; ***p < 0.01. C, LTP_HFS was evoked in both 0-HIF1a^+/− (n = 8, gray) and 10-HIF1a^+/− (n = 8, dark yellow). No difference was found when comparing LTP_HFS magnitude between 0-HIF1a^+/− and 10-HIF1a^+/− (p = 0.94). D, LTP_TBS was evoked in both 0-HIF1a^+/− (n = 6, light gray), 10-HIF1a^+/− (n = 5, light yellow), and 10-HIF1a^+/− + AP5 (n = 5, light green). No difference was found when comparing LTP_TBS magnitude of 0-HIF1a^+/− and 10-HIF1a^+/−. Representative traces illustrate baseline (black) and 60 min following HFS (colored trace). Scale bars: 0.2 mV/10 ms. In experiments using AP5, electrophysiological recordings began at 20 min before eliciting LTP (i.e., t = −20) while AP5 was applied 10 before eliciting LTP (i.e., t = −10). For all the experiment, the arrow represents the electric protocols: HFS or TBS; ***p < 0.001, **p < 0.01; N.S., p > 0.05.