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. 2020 Jun 19;7(3):ENEURO.0024-20.2020. doi: 10.1523/ENEURO.0024-20.2020

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Copyright © 2020 Arias-Cavieres et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 3. — The IH reduces the contribution of the NMDAr to fEPSP and GluN1 protein from wild-type mice but does not induce these changes in HIF1a^+/−. A, Representative traces of the fEPSP from control, IH₁₀, 0-HIF1a^+/−, and 10-HIF1a^+/− in: aCSF (black), Mg²⁺-free media (blue), and Mg²⁺-free media with AP5 (red). Scale bars: 0.4 mV/10 ms. B, top, Change in amplitude of the fEPSP from aCSF to Mg²⁺-free media. Bottom, Change in amplitude of the fEPSP from Mg²⁺-free media to Mg²⁺-free media with AP5; *p < 0.05; N.S., p > 0.05. C, left, Representative Western blottings of GluN1 and the housekeeping protein, GAPDH from control (n = 5), IH₁₀ (n = 5), 0-HIF1a^+/− (n = 5), and 10-HIF1a^+/− (n = 5). Right, Comparisons of normalized GluN1 protein expression were performed to compare experimental conditions to control. This revealed that GluN1 was reduced in IH₁₀ and unchanged in both 0-HIF1a^+/− and 10-HIF1a^+/−; *p < 0.05; N.S., p > 0.05. D, left, Representative Western blottings of PSD-95 and the housekeeping protein, GAPDH from control (n = 3), IH₁₀ (n = 3), 0-HIF1a^+/− (n = 3), and 10-HIF1a^+/− (n = 3). Right, Comparisons of normalized PSD-95 protein expression were performed to compare experimental conditions to control; *p < 0.05; N.S., p > 0.05.