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Generation of TMEM106B-deficient mice. (A and B) Illustration of mouse TMEM106B genomic locus and guide RNAs used to generate TMEM106B-deficient mice. Red box indicates deleted region. (C–E) Ablation of TMEM106B gene product was confirmed by PCR (C) and western blot analysis using antibodies specific to TMEM106B (D and E).
