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. 2020 Jul 15;8(14):e14505. doi: 10.14814/phy2.14505

FIGURE 4.

FIGURE 4

FLPK binding to FGF14 assessed by surface plasmon resonance. (a) representative SPR sensorgram of FLPK (1–100 µM) binding to FGF14. (b) average saturated binding curve for FLPK binding to FGF14 for three independent experiments. FGF14 purified protein (RU 16 000) was immobilized (using the Amine Coupling Kit, see Methods) on CM5 sensor chips, and FLPK was flown over the chip using a flow rate of 50 µl/min. Data are mean ± SEM in panels (b)