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. 2020 Jul 15;10:11674. doi: 10.1038/s41598-020-68313-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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JNK signaling activation is necessary for Zfh-1 dysregulation induced by refeeding. Percentages of testes exhibiting ectopic Zfh-1 expression (A, B) and early germ cell overproduction (C, D). (A) Ectopic Zfh-1 expression by refeeding was markedly suppressed by depletion of hep or overexpression of puc in cyst lineage cells at restrictive temperature 29 °C. (B) No suppression was observed when Gal80^ts-mediated JNK signaling inactivation was blocked at permissive temperature 25 °C. (C) Refeeding-induced early germ cell overproduction was also noticeably suppressed by puc overexpression in cyst lineage cells at 29 °C. (D) No significant suppression of early germ cell accumulation was observed at permissive temperature 25 °C. Statistics were generated using Chi-squared test.