Scanning Electron Microscopy of Cardiac-Neuron Co-cultures
(A) Exemplar image for sympathetic neurons growing in co-culture with cardiomyocytes in vitro (micrographs were colored in post-processing). Scale bar: 25 μm.
(B) Close up of a connection between neurite extension and cardiac syncytium showing connections between neurons and myocytes. Scale bar: 5 μm.
(C–F) SEM images showing stellate sympathetic neurons on cardiac monolayers. Neurons making connections to other neurons (C), wide spreading neurite extensions (D), neuron bodies and extensions make contact with myocytes (E), white asterisk showing dendritic process (E) and white arrow (F) indicating neuron body. Scale bars: (C) 50, (D) 50, (E) 10, and (F) 20 μm respectively.
See also Figure S1.