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Methods of assessing myogenic differentiation within in vitro culture systems.
| Myogenic outcome | Marker assessed | In vitro analysis method | Time after induction |
|---|---|---|---|
| Myotube formation |
|
Immunofluorescence | 3–7 days |
| Myogenic gene expression |
|
Polymerase chain reaction | 3–21 days |
| Myogenic protein production |
|
Western blotting ELISA |
7–14 days |
| Force production |
|
Electrical stimulation | 7–21 days |
| NMJs formation |
|
Immunofluorescence | 14 days |
