Fig. 5.

Autophagy-related protein 10 (ATG10) is a target of miR-221/222. a Luciferase reporter assay was used to detect the luciferase activity of wt and mut ATG10 in PTC cells. Wt and mut of the putative miR-221/222 targeting sequence in ATG10 mRNA. b, c qRT-PCR shows the expression of ATG10 protein in PTC cells following transfection. d, e WB analysis shows ATG10 protein levels, GAPDH was used as a control. The test was repeated independently in triplicates. Two-sample and other variance t-tests were used for data treatment. Error bars indicate mean ± SD. (**P < 0.01 was considered to be statistically significant; mimics, overexpression; inhibitor, interference; mimics NC, overexpression negative control; inhibitor NC, interference negative control; miR, microRNA; wt, wild-type; mut, mutant)