Figure 3.

Apparent translation processivity depends on bL31 protein. Apparent translation processivity of E. coli strains encoding both bL31 paralogs (MG), the bL31A paralog (A), the bL31B paralog (B) or none of the bL31 paralogs (ΔAB) were assayed for luminescence with The Dual-Luciferase Reporter (DLR) Assay System (Promega)³⁴. Activities of the fusion protein were measured from exponentially growing cells at 30 °C and expressed as Fluc/Rluc ratios. (a) Strains transformed with the dual luciferase fusion reporter, n = 9–15. (b) Strains containing the dual luciferase reporter complemented with a plasmid expressing bL31B (pB), bL31A (pA) or empty vector (pMOCK), n = 3–8. Statistical significance was determined by the unpaired two sample Student's t test (*P < 0.05; NS, not significant).