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. 2020 Jul 15;10:11656. doi: 10.1038/s41598-020-68249-y

Table 1.

Results of whole-genome sequencing of S. pyogenes NCTC 8198T and CCUG 4207T, from the four sequencing runs done with PacBio RSII, Illumina HiSeq 2,500 and Oxford Nanopore MinION platforms.

Platform PacBio RSII Illumina HiSeq 2500 Oxford Nanopore MinION
Run SRA accession number ERR550482 ERR550487 SRR8631872 SRR10092043
Total number of reads 163,469 163,274 10,260,942 135,020
Total yield (Mb) 420 694 1,293 1,122
Sequencing depth 219 X 362 X 675 X 586 X
Average read length (bp) 2,570 4,250 126 8,307
Read length N50 (bp) 4,555 9,809 126 14,598
Longest read (bp) 35,267 41,160 126 97,527
Average Phred quality score 2.7 3.8 35 11.5

The total number of reads, total yield (Mb), sequencing depth, average read length (bp), read length N50 (bp), longest read (bp) and average Phred quality score are shown for each sequencing run. The SRA accession number of each run is indicated.