Fig. 5. CLP mice present adverse metabolic changes.

a Twenty-four hours post-CLP, blood pH was significantly decreased with sepsis severity. b OCR measurements of splenocytes from naive and CLP mice showed aberrant mitochondrial respiration, predominantly in severely septic mice (MSS > 10), which was manifested primarily by c a decreased coupling efficiency. d ECAR measurements of splenocytes from naive and CLP mice showed only mild changes in the general glycolytic function, which was slightly increased in moderately septic mice (MSS 7–8.5). e The glycolytic reserve of splenocytes in this assay was significantly decreased in severely septic mice (MSS > 14). f Blood lactate concentration was slightly lower in CLP mice. Data in (a), (c), (e), and (f) are presented as the median within the inter-quartile range (IQR); mean values are marked with a ‘+’ sign; error bars represent the minimum and maximum; data in (b) and (d) are presented as the mean ± standard deviation. Group sizes (N) are indicated below their respective legends; *P ≤ 0.01, **P ≤ 0.001, ***P ≤ 0.0001 by the Kruskal–Wallis nonparametric ANOVA, with multiple-comparisons adjusted using Dunn’s test. P values above the bars indicate the significant differences from the control group, and those above the brackets indicate the significant differences between the two other groups.