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. 2020 Jul 15;10:11681. doi: 10.1038/s41598-020-68574-2

Figure 2.

Figure 2

Piperine reduces β-catenin nuclear localization. Immunostainings of β-catenin of RKO cells treated with (A–A″) L-cell conditioned medium, with (B–B″) L-Wnt3a conditioned medium co-treated with DMSO or with (C–C″) piperine 100 µM. (D–D″) XAV939 was used as a positive control for Wnt signaling inhibition. (E) Graph of β-catenin positive nuclei percentage quantification. (F) Immunoblot for β-catenin of HCT116 cells untreated or treated with DMSO or 50, 100 μM piperine for 24 h. The raw immunoblot data is shown in Supplementary Figure S4. Scale bar = 38 μm. *p < 0.05, **p < 0.01.