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Mean percentage cell viability of (A) hMSC, (B) U2OS cells, and (C) U2OS spheroids after 24 h incubation with varying concentrations of free DOXO (0–200 μM) in the respective growth media at 37°C, used to identify the IC50 value of DOXO in these cell types (n = 3 replicas, ±SD) (D).
