Figure 2.

Real-Time, Longitudinal Assessment of Proliferation Kinetics in Live hiPSCs Reveals Synchronized Cell Divisions

(A) Representative images of 24-h time lapses (121 × 12 min frames spanning the period of 3–4 days after plating, related to Videos S1, S2, and S3). One frame every 6 h during a time lapse is shown. Scale bars, 20 μm.

(B) Representative example of tracking single parental cell (P) just before it divides (t = 0 h), its daughters (D1 and D2 sisters), and granddaughters (D1.1, D1.2, D2.1, and D2.2). Scale bars, 10 μm.

(C) Quantification of cell-cycle duration of tracked sister cells, cells from the same lineage (common ancestor cell at time of plating), and all cells measured.

(D) Difference from mean cell division time point among sister cells, cells from the same lineage, and all cells. ^∗p < 0.05 versus all cells.