Figure 5.

Rainbow hiPSCs Have the Capacity to Differentiate to All Germ Layers and Maintain Reporter Expression

(A–C) All differentiations started with high-dose Cre-treated rainbow hiPSCs replated at day 0. Spectral analysis of color diversity, rainbow reporter signal, and respective cell-type-specific marker expression in (A) hepatoblasts, (B) cortical neurons, and (C) cardiac myocytes. Scale bars, 20 μm.

(D) Repeat imaging of rainbow-labeled hiPSCs to cardiomyocytes starting from the first day of differentiation through 1 week (days 1–7). Scale bars, 100 μm.