FIG 10.

Synthetic growth defects of prf1-Δ345 spt5, prf1-Δ458 spt5, and prf1-Δ472 double mutants. (A) Fivefold serial dilutions of strains were spotted onto agar plates containing control medium (YES), medium with 0.01% MMS, or rich medium with 15 mg/ml TBZ. Strains are indicated on the left. All experiments were repeated at least 3 times, and representative pictures are shown. (B, top) Immunoblots of whole-cell extracts from the indicated strains. (Bottom) Ratios of TAP/Rpb1 signals for each sample were normalized to that in prf1-TAP spt5⁺. Error bars denote standard errors of the means from 3 independent experiments. One-way ANOVA was conducted across all prf1⁺ strains within an spt5⁺ background followed by two-sided t tests with Bonferroni correction between each prf1⁺ mutant strain and the prf1-TAP strain in the same spt5⁺ background. **, P ≤ 0.01.