FIG 12.

The PAF complex is necessary for Prf1 recruitment to chromatin. TAP tag ChIP was performed on the indicated strains and quantified by qPCR using the indicated primers in act1⁺ (A), nup189⁺ (B), and spb354⁺ (C); percent IP values were normalized to the input for each corresponding strain and primer pair. The length of the gene (in base pairs) and positions of PCR amplicons are shown in the diagram at the top. Error bars denote standard errors of the means from 3 independent experiments. Two-way ANOVA was conducted followed by two-sided t tests with Bonferroni correction between each strain and the untagged control within a specific primer pair. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001.