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Synthetic growth defects of prf1-R227A spt5 double mutants. Fivefold serial dilutions of strains were spotted onto agar plates containing control medium (YES), medium with 0.01% methyl methanesulfonate (MMS), or rich medium with 15 mg/ml thiabendazole (TBZ). Strains are indicated on the left. All experiments were repeated at least 3 times, and representative pictures are shown.
