FIG 8.

Synthetic growth defects of prf1-R262E spt5 and prf1-R296E spt5 double mutants. (A) Fivefold serial dilutions of strains were spotted onto agar plates containing control medium (YES), medium with 0.01% MMS, or rich medium with 15 mg/ml TBZ. Strains are indicated on the left. All experiments were repeated at least 3 times, and representative pictures are shown. (B) Immunoblots of whole-cell extracts from the indicated strains (top). Ratios of TAP/Rpb1 signals for each sample were normalized to that in prf1-TAP spt5⁺. Error bars denote standard errors of the means from 3 independent experiments (bottom). One-way ANOVA was conducted across all prf1⁺ strains within an spt5⁺ background followed by two-sided t tests with Bonferroni correction between each prf1⁺ mutant strain and the prf1-TAP strain in the same spt5⁺ background.