TABLE 1.
S. pombe strains used in this study
| Strain | Genotype | Reference or source |
|---|---|---|
| JT204 | h− ade6-M216 | 30 |
| JT202 | h− prf1-TAP::kanMX6 ade6 | 13 |
| JT362 | h+ leu1-32 ura4-D18 his3-D1 ade6-M210 | R. Fisher |
| JT98-1 | h− htb1-K119R::kanMX6 ade6 leu1-32 ura4-D18 | 30 |
| JT113-2 | h− prf1Δ::hphMX4 ade6 | 16 |
| JT435 | h− tpr1-TAP::kanMX6 | 16 |
| JT924 | h− prf1-R227A-TAP::kanMX6 ade6-M216 | This study |
| JT925 | h− prf1-R296E-TAP::kanMX6 ade6-M216 | This study |
| JT926 | h− prf1-R262E-TAP::kanMX6 ade6-M216 | This study |
| JT921 | h− prf1-Δ345-TAP::kanMX6 ade6-M216 | This study |
| JT923 | h− prf1-Δ458-TAP::kanMX6 ade6-M216 | This study |
| JT933 | h− prf1-Δ472-TAP::kanMX6 ade6-M216 | This study |
| JT956 | h? prf1-Δ345-TAP::kanMX6 spt5(7)::ura4+ ade6-M216 ura4-D18 leu1-32? his3-D1? | This study |
| JT957 | h? prf1-Δ345-TAP::kanMX6 spt5-T1A(7)::ura4+ ade6-M216? ura4-D18 leu1-32? his3-D1? | This study |
| JT958 | h? prf1-Δ345-TAP::kanMX6 spt5-T1E(7)::ura4+ ade6-M216? ura4-D18 leu1-32? his3-D1? | This study |
| JT959 | h? prf1-Δ345-TAP::kanMX6 spt5-ΔC::ura4+ ade6-M216? ura4-D18 leu1-32? his3-D1? | This study |
| JT960 | h? prf1-Δ458-TAP::kanMX6 spt5(7)::ura4+ ade6-M216 ura4-D18 leu1-32? his3-D1? | This study |
| JT961 | h? prf1-Δ458-TAP::kanMX6 spt5-T1A(7)::ura4+ ade6-M216? ura4-D18 leu1-32? his3-D1? | This study |
| JT962 | h? prf1-Δ458-TAP::kanMX6 spt5-T1E(7)::ura4+ ade6-M216? ura4-D18 leu1-32? his3-D1? | This study |
| JT965 | h? prf1-Δ458-TAP::kanMX6 spt5-ΔC::ura4+ ade6-M216? ura4-D18 leu1-32? his3-D1? | This study |
| JT977 | h? prf1-R296E-TAP::kanMX6 spt5(7)::ura4+ ade6-M216 ura4-D18 leu1-32 his3-D1 | This study |
| JT978 | h? prf1-R296E-TAP::kanMX6 spt5-T1A(7)::ura4+ ade6-M216? ura4-D18 leu1-32 his3-D1 | This study |
| JT979 | h? prf1-R296E-TAP::kanMX6 spt5-T1E(7)::ura4+ ade6-M216? ura4-D18 leu1-32 his3-D1 | This study |
| JT980 | h? prf1-R296E-TAP::kanMX6 spt5-ΔC::ura4+ ade6-M216? ura4-D18 leu1-32 his3-D1 | This study |
| JT981 | h? prf1-R262E-TAP::kanMX6 spt5(7)::ura4+ ade6-M216 ura4-D18 leu1-32? his3-D1? | This study |
| JT982 | h? prf1-R262E-TAP::kanMX6 spt5-T1A(7)::ura4+ ade6-M216 ura4-D18 leu1-32? his3-D1? | This study |
| JT983 | h? prf1-R262E-TAP::kanMX6 spt5-T1E(7)::ura4+ ade6-M216 ura4-D18 leu1-32? his3-D1? | This study |
| JT984 | h? prf1-R262E-TAP::kanMX6 spt5-ΔC::ura4+ ade6-M216 ura4-D18 leu1-32? his3-D1? | This study |
| JT966 | h? prf1-Δ472-TAP::kanMX6 spt5(7)::ura4+ ade6-M216 ura4-D18 leu1-32? his3-D1? | This study |
| JT986 | h? prf1-Δ472-TAP::kanMX6 spt5-T1A(7)::ura4+ ade6-M216? ura4-D18 leu1-32? his3-D1? | This study |
| JT987 | h? prf1-Δ472-TAP::kanMX6 spt5-T1E(7)::ura4+ ade6-M216? ura4-D18 leu1-32? his3-D1? | This study |
| JT988 | h? prf1-Δ472-TAP::kanMX6 spt5-ΔC::ura4+ ade6-M216? ura4-D18 leu1-32? his3-D1? | This study |
| JT997 | h? prf1-R227A-TAP::kanMX6 spt5(7)::ura4+ ade6-M216 ura4-D18 leu1-32? his3-D1? | This study |
| JT998 | h? prf1-R227A-TAP::kanMX6 spt5-T1A(7)::ura4+ ade6-M216 ura4-D18 leu1-32? his3-D1? | This study |
| JT999 | h? prf1-R227A-TAP::kanMX6 spt5-T1E(7)::ura4+ ade6-M216 ura4-D18 leu1-32? his3-D1? | This study |
| JT1000 | h? prf1-R227A-TAP::kanMX6 spt5-ΔC::ura4+ ade6-M216? ura4-D18 leu1-32? his3-D1? | This study |
| JT1040 | h? prf1-TAP::kanMX6 spt5(7)::ura4+ ade6-M216 ura4-D18 leu1-32? his3-D1? | This study |
| JT1041 | h? prf1-TAP::kanMX6 spt5-T1A(7)::ura4+ ade6-M216 ura4-D18 leu1-32? his3-D1? | This study |
| JT1042 | h? prf1-TAP::kanMX6 spt5-T1E(7)::ura4+ ade6-M216 ura4-D18 leu1-32? his3-D1? | This study |
| JT1043 | h? prf1-TAP::kanMX6 spt5-ΔC::ura4+ ade6-M216 ura4-D18 leu1-32? his3-D1? | This study |
| JT340 | h− spt5(7)::ura4+ leu1-32 ura4-D18 his3-D1 ade6-M210 | This study |
| JT341 | h− spt5-T1A(7)::ura4+ leu1-32 ura4-D18 his-D1 ade6 | This study |
| JT342 | h− spt5-T1E(7)::ura4+ leu1-32 ura4-D18 his-D1 ade6 | This study |
| JT343 | h− spt5-ΔC::ura4+ leu1-32 ura4-D18 his3-D1 ade6 | This study |
| JT771 | h+ spt5(7)::ura4+ leu1-32 ura4-D18 his3-D1 ade6-M210 | 12 |
| JT772 | h+ spt5-T1A(7)::ura4+ leu1-32 ura4-D18 his-D1 ade6-M210? | 12 |
| JT773 | h+ spt5-T1E(7)::ura4+ leu1-32 ura4-D18 his-D1 ade6-M210? | 12 |
| JT774 | h+ spt5-ΔC::ura4+ leu1-32 ura4-D18 his3-D1 ade6-M210? | 12 |
| JT483 | h? paf1-TAP::kanMX6 | This study |
| JT501 | h? prf1Δ::hphMX4; paf1-TAP::kanMX6 | This study |
| JT625 | h? tpr1Δ::kanMX5; prf1-TAP::kanMX6 ade6-M216 ura4-D18 leu1-32 his− | This study |