Table 2.
Levels of Pro-immune Molecules Secreted from the Bone Marrow Myeloma Tumor Microenvironment after 3 or 24 h of Treatment
| Vehicle (1× PBS) | BM | PBMCs | MYXV | BM + MYXV | PBMCs + MYXV | |
|---|---|---|---|---|---|---|
| After 3 h | ||||||
| IFN-γ (Th1) | 15.5 ± 4.5 | 13.0 ± 0.0 | 20.0 ± 5.0 | 21.5 ± 7.3 | 103.7 ± 6.3 | 17.7 ± 1.3 |
| IP-10 (Th1) | 735.0 ± 222.0 | 1,082.5 ± 112.5 | 895.5 ± 106.0 | 981.0 ± 125.4 | 4,034.8 ± 78.3 | 1,676.3 ± 223.7 |
| IL-12p70 (Th1) | 3.5 ± 0.0 | 3.7 ± 0.2 | 4.0 ± 0.5 | 3.8 ± 1.4 | 6.1 ± 0.3 | 5.0 ± 0.3 |
| IL-4 (Th2) | 11.3 ± 1.5 | 12.8 ± 4.5 | 15.5 ± 0.7 | 10.5 ± 2.5 | 15.6 ± 3.8 | 10.5 ± 2.2 |
| IL-5 (Th2) | 7.0 ± 4.0 | 7.2 ± 0.2 | 10.7 ± 3.2 | 7.0 ± 3.1 | 11.7 ± 4.2 | 10.7 ± 0.9 |
| TNF-α | 53.5 ± 14.0 | 60.7 ± 1.2 | 53.3 ± 1.7 | 47.5 ± 5.5 | 83.5 ± 8.7 | 65.2 ± 2.3 |
| IL-1α | 38.7 ± 6.2 | 35.5 ± 8.0 | 30.2 ± 3.7 | 34.8 ± 6.0 | 66.2 ± 12.2 | 34.8 ± 3.4 |
| IL-1β | 112.0 ± 43.0 | 52.7 ± 8.7 | 83.0 ± 12.0 | 73.3 ± 5.2 | 167.2 ± 32.2 | 81.0 ± 7.0 |
| GM-CSF | 3.0 ± 0.0 | 2.5 ± 0.5 | 35.5 ± 0.5 | 3.3 ± 0.7 | 4.7 ± 0.3 | 2.7 ± 0.3 |
| MIP-1α | 341.0 ± 179.0 | 371.2 ± 2.2 | 301.5 ± 69.5 | 449.8 ± 43.0 | 1,515.8 ± 238.2 | 380.7 ± 51.8 |
| IL-6 | 17.5 ± 2.5 | 14.7 ± 1.2 | 12.0 ± 2.0 | 14.3 ± 1.9 | 20.2 ± 0.4 | 13.0 ± 1.7 |
| After 24 h | ||||||
| IFN-γ (Th1) | 20.0 ± 2.1 | 10.0 ± 1.5 | 9.0 ± 1.5 | 573.3 ± 225.2 | 196.2 ± 28.9 | 170.0 ± 5.6 |
| IP-10 (Th1) | 1,110.0 ± 71.0 | 741.3 ± 64.6 | 714.3 ± 51.6 | 2,080.0 ± 106.0 | 1,376.0 ± 187.3 | 1,670.3 ± 60.4 |
| IL-12p70 (Th1) | 2.5 ± 0.0 | 2.8 ± 0.9 | 2.2 ± 0.3 | 7.4 ± 1.1 | 5.1 ± 0.4 | 4.1 ± 0.2 |
| IL-4 (Th2) | 6.8 ± 1.5 | 5.3 ± 1.0 | 7.3 ± 1.2 | 21.0 ± 4.2 | 9.7 ± 0.7 | 14.2 ± 1.7 |
| IL-5 (Th2) | 4.5 ± 0.5 | 4.0 ± 0.3 | 5.0 ± 0.6 | 10.7 ± 1.6 | 7.4 ± 0.5 | 9.6 ± 0.4 |
| TNF-α | 32.0 ± 2.5 | 258 ± 3.8 | 22.2 ± 4.5 | 44.2 ± 6.1 | 812.6 ± 124.1 | 24.5 ± 2.1 |
| IL-1α | 41.0 ± 10.5 | 19.5 ± 2.3 | 14.5 ± 3.0 | 69.4 ± 20.0 | 35.8 ± 4.9 | 14.1 ± 1.1 |
| IL-1β | 44.0 ± 18.0 | 19.5 ± 2.3 | 127.3 ± 24.4 | 124.0 ± 80.6 | 35.8 ± 4.9 | 138.9 ± 5.7 |
| GM-CSF | 1.0 ± 0.0 | 1.3 ± 0.3 | 1.3 ± 0.3 | 7.2 ± 0.9 | 2.5 ± 0.5 | 4.3 ± 1.2 |
| MIP-1α | 422.0 ± 111.0 | 128.5 ± 16.9 | 121.7 ± 14.5 | 2,966.0 ± 853.3 | 812.6 ± 124.1 | 651.0 ± 71.5 |
| IL-6 | 4.5 ± 0.5 | 8.7 ± 5.2 | 6.2 ± 2.4 | 9.3 ± 1.3 | 9.3 ± 1.3 | 9.5 ± 1.5 |
Means ± standard deviation (SD) of levels of the secreted molecules are shown.