Table 1.
Subcellular Localization, Numbers, and Enrichment of Ribosomal Proteins (RPs) Regulated at the Transcript Level during Acclimation
| Subcellular Localization & Subunit | HL |
Heat |
Cold |
Total | |||
|---|---|---|---|---|---|---|---|
| # (Up/Down) | FE | # (Up/Down) | FE | # (Up/Down) | FE | ||
| Cytosol | |||||||
| 40S | 20 (0/20) | 0.83 | 28 (2/26) | 1.01 | 56 (56/0) | 1.10 | 101 |
| 60S | 41 (2/39) | 1.04 | 39 (0/39) | 0.86 | 110 (110/0) | 1.32∗ | 165 |
| All | 61 (2/59) | 0.96 | 67 (2/65) | 0.91 | 166 (166/0) | 1.24∗ | 266 |
| Chloroplast | |||||||
| 30S | 9 (0/9) | 1.44 | 10 (0/10) | 1.40 | 3 (2/1) | 0.23∗ | 26 |
| 50S | 16 (0/16) | 1.31 | 23 (0/23) | 1.64∗ | 9 (7/2) | 0.35∗ | 51 |
| All | 25 (0/25) | 1.35∗ | 33 (0/33) | 1.56∗ | 12 (9/3) | 0.31∗ | 77 |
| Mitochondrion | |||||||
| 30S | – | 0.00∗ | – | 0.00∗ | – | 0.00∗ | 13 |
| 50S | 1 (0/1) | 0.60 | – | 0.00 | 5(5/0) | 1.42 | 7 |
| All | 1 (0/1) | 0.21∗ | 0 (0/0) | 0.00∗ | 5 (5/0) | 0.50∗ | 20 |
| Total | |||||||
| 87 (2/85) | 100 (2/98) | 183 (180/3) | 363 | ||||
Transcriptional super-hubs for RPs were classified according to the subcellular localization of the corresponding ribosomal subunits based on MapMan (Thimm et al., 2004). The total number of transcripts in each category and the fold enrichment (FE) relative to the expected frequency of RPs are listed. The regulatory trend (up- or down-regulation) refers to changes in the transcript levels at 4 days of acclimation compared with the initial time point. Statistical significance of the enrichment according Fisher's two-sided exact test (p ≤ 0.05) is indicated by an asterisk.