Figure 2.
Glucose Upregulates MCT4 Expression in the Aerobic Glycolysis-Preference NSCLC Cell Subtype
(A) Intracellular lactate concentrations of the ten NSCLC cell lines were measured by a lactate assay kit. 3 × 105 cells were collected for each cell line and the concentration was measured as described in the Materials and Methods. Data represent mean and SD. n = 3. (B) The protein levels of MCT1 to 4 in the ten NSCLC cell lines were detected in whole-cell lysates by immunoblotting. (C) The mRNA level of MCT4 (SLC16A3) in the ten NSCLC cell lines was detected by qRT-PCR and the graph indicated the levels in each cell line relative to that in PC9. Data represent mean and SD. n = 3. (D) PC9, CL97, CL1-5, and Hop62 cells were treated with 0, 5, or 10 mM of glucose for 24 h. The protein levels of MCT4 and Sp1 were detected in whole-cell lysates by immunoblotting. (E) PC9, CL97, CL1-5, and Hop62 cells were treated with 0, 5, or 10 mM of glucose for 24 h. The mRNA level of MCT4 (SLC16A3) was detected by qRT-PCR. Data represent mean and SD. n = 3. (F) The mRNA level of MCT4 (SLC16A3) was detected by qRT-PCR in PC9, CL97, CL1-5, and Hop62 cells overexpressing Neo or Sp1. Data represent mean and SD. n = 3.