FIGURE 4.

TA treatment attenuated MPTP-induced decrease in tyrosine hydroxylase (TH) expression in mice. (A,B) Western blot analysis of the level of TH in the mouse midbrain and striatum. The relative optical density of TH was normalized by the internal loading control GAPDH. (C) Immunofluorescence staining with anti-TH antibody in the substantia nigra pars compacta (SNc). Scale bar: 200 μm. One-way ANOVA followed by post hoc Bonferroni test: ^##p < 0.01, ^###p < 0.001 compared with the control group; *p < 0.05, ***p < 0.001 compared with the MPTP group (n ≥ 3). Data are shown as mean ± SEM.