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. 2020 Jul 9;36:101642. doi: 10.1016/j.redox.2020.101642

Fig. 7.

Fig. 7

Vinculin (VCL) affected PI3K/AKT/eNOS and ERK1/2 signaling pathways.(A–E) Immunoblots and dot plots show that knockdown of vinculin inhibited the expressions of PI3K, AKT, eNOS and ERK1/2 as well as the phosphorylations of AKT, eNOS, and ERK1/2 in cultured endothelial cells (ECs) in all groups, but increased the expression of caveolin-1 in cultured ECs in control- and nHDL-treated groups. NC, negative control, * vs. control (NC-siRNA); # vs. nHDL (NC-siRNA); & vs. dHDL (NC-siRNA), p < 0.05 (n = 10). (F–J) Immunoblots and dot plots show that overexpression of vinculin increased the expressions of PI3K, AKT, eNOS and ERK1/2 as well as the phosphorylations of AKT, eNOS and ERK1/2, but decreased the expression of caveolin-1 in cultured ECs in all groups. NC, negative control; OE, overexpression. * vs. Empty-NC; # vs. Empty-NC + nHDL; & vs. Empty-NC + dHDL, p < 0.05 (n = 6–8).