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. 2020 Jul 16;883:173374. doi: 10.1016/j.ejphar.2020.173374

Fig. 1.

Fig. 1

Regulatory roles of miR-122-5p mimic in cellular autophagy and migration in rat AFs. (A) Representative images of immunofluorescence staining of vimentin (green) and α-SMA (red) in rat AFs. (B) The relative mRNA level of miR-122-5p in rat AFs after the transfection of miR-122-5p mimic. U6 was used as an endogenous control. (C) Immunofluorescence staining array of LC3 (green) and p62 (red) in rat AFs pre-treated with ATII, NC, miR-122-5p mimic, AICAR, and Rapa, respectively. (D-E) Representative western blots images and quantification in rat AFs after stimulation with miR-122-5p mimic. GAPDH was used as an endogenous control. (F-G) In vitro wound healing images with quantification in rat AFs in 0 h and 24 h, respectively. n = 3–4 for each group except for B where n = 6. **, P < 0.01, ***, P < 0.001 compared with control or NC group; #, P < 0.05, ##, P < 0.01 compared with ATII or ATII + NC group. A.U., arbitrary units; R.E., relative expression; NC, negative control; AFs, adventitial fibroblasts; ATII, angiotensin II; AICAR, 5-amino-4-imidazole carboxamide riboside; Rapa, rapamycin; α-SMA, α-smooth muscle actin; DAPI, 4′,6′-diamidino-2-phenylindole.