Fig. 2.

Regulatory roles of miR-122-5p mimic in oxidative stress and apoptosis in rat AFs. (A-B) Representative dihydroethidium staining images with quantification in rat AFs in the absence and presence of ATII, NC, miR-122-5p mimic, NAC, AICAR, and Rapa, respectively. (C-D) Percentage of apoptotic cells by flow cytometry. (E-F) Representative western blots with quantification to examine the protein levels of caspase-3, bax, and bcl-2. GAPDH was used as an endogenous control. n = 3–4 for each group. **, P < 0.01 compared with control group; #, P < 0.05, ##, P < 0.01 compared with ATII or ATII + NC group. A.U., arbitrary units; AFs, adventitial fibroblasts; NC, negative control; ATII, angiotensin II; NAC, N-acetyl-L-cysteine; AICAR, 5-amino-4-imidazole carboxamide riboside; Rapa, rapamycin; NA, no statistical significance.