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Characterizations of MSCs. (a) Morphologies of primary and subcultured (P4) MSCs. (b) Flow cytometry analysis of the immunophenotypes of P4 MSCs. MSCs were positive for CD90 and CD105, but negative for CD45 and CD34 (n = 3). (c) Differentiation potentials of MSCs. Adipogenesis and osteogenesis of MSCs were confirmed by Oil Red O staining and Alizarin Red S staining after culturing in corresponding induction or control medium. Bar = 100 μm.
