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Hepatocyte MANF deletion activates the downstream genes of NF-κB in alcoholic liver injury. (a) Liver tissues were subjected to immunostaining with anti-TNF-α and IL-1β antibodies. Representative images are shown. Scale bar = 40 μm. (b–g) The relative mRNA levels of TNF-α (b), IL-1β (c), IL-6 (d), MCP-1 (e), MIP-1α (f), and MIP-1β (g) were detected by real-time PCR analysis. The values are expressed as means ± SEM (n = 6–8). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.
