FIG 1.

Previously identified antigenic sites on hantavirus glycoprotein Gn or Gc. (a and b) Neutralizing MAbs and epitopes for serum antibody recognition identified by peptide scanning and generation of escape mutant viruses are mapped on the crystal structure of Puumala virus Gn protein (PDB: 5FXU) in the tetrameric form on the surface of the virion. Gn is shown as a tetramer from the top view (a) or dimer from the side view (b). (c) Epitopes and escape mutations also are mapped to the linear genome of Gn. The signal peptide (SP) or transmembrane domain (TM) is highlighted in yellow or gray, respectively. (d and e) Previously identified neutralizing MAb and serological epitopes identified by peptide scanning and generation of escape mutant viruses are mapped on the crystal structure of Puumala virus Gc in the postfusion form of trimer (PDB: 5J9H). Gc is shown as a trimer (d) or protomer (e) to demonstrate epitopes that are inaccessible in the trimer. (f) Epitopes and escape mutations also are mapped to the linear genome of Gc. Domains I, II, and III are indicated in red, yellow, or blue, respectively, and the fusion loop is indicated in orange. Spheres indicating epitopes are color coded as follows: green, PUUV MAbs (5A2, 1C9, 4G2); purple, HTNV MAbs (M7, Y1, Y5, Y7, Y22); orange, sera from convalescent Sin Nombre patients; teal, sera from convalescent Puumala patients; pink, sera from convalescent ANDV patients. Escape mutations are indicated by the blue (anti-HTNV MAbs) or orange (anti-ANDV MAbs) bars.