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clbR promoter activity depends on iron availability. Levels of growth (OD600) and relative luminescence (RLU/OD600) of E. coli strain M1/5 λ-attB::5VNTR-clbRp-lux were measured in LB (A) and M9+CAS medium (B) depending on iron availability. The availability of ferric iron was altered by the addition of either 100 μM FeCl3 or 0.2 μM deferroxamin. Median values of results from biological and technical triplicates are shown with standard deviations.
