FIG 9.

Impact of clbR and clbQ expression on global gene expression of E. coli M1/5 rpsLK42R at the transcriptome and proteome levels. We compared the levels of impact of clbR or clbQ overexpression as well as of clbR or clbQ deletion on global gene expression of E. coli M1/5 rpsLK42R at the transcriptional and translational levels by RNA-seq and proteome analysis, respectively. Three biological replicates were pooled before aliquots were used for RNA-seq or mass spectrometry-based proteome analysis. The expression profiles of genes (A) and proteins (B) displaying a log₂ fold change value of less than or equal to −2 or greater than or equal to +2 in the different E. coli M1/5 variants relative to the wild type were subjected to cluster analysis. The gene/protein designations are indicated as well as groups of genes/proteins with similar expression patterns. Groups of genes or proteins that displayed an enrichment of functionally related proteins are marked in color, and the corresponding Gene Ontology (GO) term is given.