Table 2.
Various molecular approaches for the detection of genetic aberrations in cfDNA
| Technique | Sensitivity | Aberrations detected | |
|---|---|---|---|
| qPCR | ARMS-Scorpions PCR | 0.05-0.1% | point mutations |
| Clamping PCR | 0.1-1% | point mutations | |
| TaqMan | 0.1-1% | point mutations | |
| Digital PCR | Beaming | 0.01% | point mutations |
| ddPCR | 0.001% | point mutations | |
| Targeted DNA sequencing | TAm-Seq | >2% | Point mutations and structural alterations in gene |
| SAFE-SeqS | 0.1% | Point mutations and structural alterations in gene | |
| CAPP-Seq | 0.01% | Point mutations and structural alterations in gene | |
| Whole genome sequencing (WES) | Digital karyotyping | 0.001% | Genome-wide copy-number changes |
| PARE | 0.001% | Genome-wide rearrangements | |