| Antibodies |
| Ly6G (clone: 1A8) |
Biolegend |
Cat. # 127609, RRID:AB_1134162 |
| F480 (clone BM8) |
Ebioscience |
Cat. # 47-4801-82 RRID:AB_2735036 |
| CD11c (N148) |
Ebioscience |
Cat # 11-0114-82 RRID:AB_464940 |
| CD11b (M1/70) |
Ebioscience |
Cat # 25-0112-82 RRID:AB_469588 |
| MHCII (M5/114.15.2) |
Ebioscience |
Cat # 12-5321-82 AB_465928 |
| CD45 (clone 30-F11) |
Biolegend |
Cat # 103137 RRID:AB_2561392 |
| H2A.X histone antibody |
Abcam |
Cat#: Ab188819 RRID:AB_10672053 |
| Mouse anti-MPO |
Abcam |
Cat # Ab9535 RRID:AB_307322 |
| Neutrophil Elastase 680 FAST Fluorescent
Imaging Agent |
PerkinElmer |
Cat # NEV11169 |
| Ghost Red Live/dead |
Tonbo |
Cat # 13-0871-T100 |
| IgGA control |
Abcam |
Cat # ab37415 RRID:AB_2631996 |
| MOA (Marasmium oreades
agglutinin)-TRITC |
EY Labs |
Cat # R-9001-1 |
| Anti-Psl (Psl0096) |
(DiGiandomenico et al., 2012) |
NA |
| Anti-PcrV (V2L2) |
(Warrener et
al., 2014) |
NA |
| Bispecific (MEDI3902) |
(DiGiandomenico
et al., 2014) |
NA |
| Bacterial Strains |
|
Pseudomonas aeruginosa PAO1
WT GFP |
Shawn Lewenza |
NA |
|
Pseudomonas aeruginosa PAO1
Δpsl GFP |
Created in this study |
NA |
|
Pseudomonas aeruginosa PAO1
ΔpcrV GFP |
Created in this study |
NA |
| PAO1 dexos GFP |
Created in this study |
NA |
| E. coli JM109 |
Promega |
Cat# (P9751) |
| Experimental Models:
Organisms/Strains |
| C57BL/6 wild-type |
Jackson |
#664 |
| B6.129S4-C3,tm1Crr./J |
Jackson |
#3641 |
| Catchup Ly6G reporter mice |
Hasenberg et
al., 2015 |
NA |
| Peptidyl arginine deiminase, type IV
(PAD4−/−) |
Gift from Kerri A. Mowen, La Jolla
California |
NA |
| Cathepsin C knockout mice (Cstc−/−) |
GalxoSmithKline |
NA |
| Neutrophil elastase knockout (NE−/−) |
Jackson |
#6112 |
| Fc R gamma receptor knockout mice
(FcRγ−/−) |
Taconic |
#583 |
| Critical Commercial Assays |
| Meso Scale Multiplex assay Kits |
Proinflammatory panel |
K152QOD |
| Plasmids |
| CTX-Ptac-GFP |
Wyckoff and
Wozniak, 2001 |
NA |
| pPM297 |
Wyckoff and
Wozniak, 2001 |
NA |
| Software and Algorithms |
| Prism |
GraphPad |
https://www.graphpad.com/scientific-software/prism/ |
| Volocity |
Quorum |
http://quorumtechnologies.com/index.php/component/content/article/31-volocity-software/31-volocity-quantitation |
| FIJI |
ImageJ |
https://fiji.sc/ |
| Leica SP8 software |
Leica |
https://www.leica-microsystems.com/products/ |
| FlowJo |
Tree Star |
https://www.flowjo.com/ |
| Other. |
| 0.3% Tobrex (3mg/mL tobramycin eye
ointment) |
Canadian distributors of veterinary
products |
NA |
| Eye ointment (no antibiotics) |
VWR |
NA |
