Figure 3.

Detection of WFA⁺-M2BP [139]. (A) Structure of WFA⁺-M2BP. WFA⁺-M2BP, which was recently shown to be a liver fibrosis glycobiomarker with a unique fibrosis-related glycoalteration, has multibranching and sialylated N-glycans. WFA lectin binds specifically to the glycosylation isomer. (B) Quantification of serum WFA⁺-M2BP. WFA⁺-M2BP quantification is measured based on a lectin-antibody sandwich immunoassay using a fully-automatic immunoanalyzer HISCL-2000i (Sysmex Corp., Hyogo, Japan). The lectin-binding WFA⁺-M2BP recognizes ALP-labelled antibody in a chemiluminescence enzyme immunoassay (CLEIA). Every reaction is accustomed to the platform during the automatic assay, which is finished after 17 minutes. M2BP, Mac2 binding protein; M2BPGi, Mac-2-binding protein glycosylation isomer; ALP, alkaline phosphatase; WFA⁺-M2BP, Wisteria floribunda agglutinin-positive Mac-2 binding protein.