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. Author manuscript; available in PMC: 2020 Jul 16.
Published in final edited form as: Mutat Res. 2011 Mar 3;714(1-2):1–10. doi: 10.1016/j.mrfmmm.2011.02.006

Fig. 2.

Fig. 2.

Mutation of serines 513 or 519 to alanine in Cdc25A prevents proteasome-mediated degradation following IR treatment. HT1080 cells were transfected with pCDNA3 plasmid control, pcDNA3-myc-Cdc25A-WT, and with each of the pcDNA3-myc-Cdc25A mutants or left non-transfected (NT), and were subjected to IR treatment or left unchallenged. Cell lysates were probed with antibody to Myc. An incubation control where cells were taken out of the incubator while other cells were being treated with IR was also included. Beta-actin was used as the loading control.