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Thymocytes derived from Plk3−/− mice have a compromised G1/S checkpoint and are more resistant to degradation of Cdc25A than wildtype cells following DNA damage. Thymocytes derived from Plk3+/+ and Plk3−/− mice were left untreated or subjected to treatment with etoposide. (A) The Cdc25A level was analyzed by Western blot. (B) Cell cycle profile was determined by flow cytometry.
