Figure 1. Inhibited Myh11 could increase cell proliferation and inhibit cell apoptosis in TAA.

(A) Expression of Myh11 was assessed by qRT-PCR assay in mouse aortic SMCs (MOVAS and A10), which were treated with H₂O₂ (800 μmol/l) at 0, 8, 16, 24 h. (B) Inhibition efficiency of Myh11 was detected via qRT-PCR assay in untreated MOVAS and A10 cells, and in H₂O₂ (800 μmol/l) treated MOVAS and A10 cells for 24 h. (C,D) Immunofluorescence assay (scale bar = 20 μm) and colony formation assay investigated the cell proliferation of MOVAS and A10 cells when Myh11 was inhibited. (E,F) TUNEL assay (scale bar = 200 μm) and flow cytometry analysis searched the apoptosis situation of MOVAS and A10 cells treated with H₂O₂ (800 μmol/l) for 24 h. *P<0.05, **P<0.01.