Fig. 5.

Ghrelin and GH in combination inhibit PD-1 and increase HLA-DR expression in the spleen of aged rats with sepsis. Rats were subjected to sham or CLP operation and treated with vehicle (normal saline) or GG (ghrelin 80 nmol/kg, GH 50 μg/kg) at 5 h after CLP. In an additional group of rats, vagotomy was performed at the time of CLP and treated with GG at 5 h after CLP. Spleens were harvested at 20 h after CLP. The expression of PD-1 and HLA-DR in the spleen were evaluated by immunohistochemical staining. The representative images are shown. Each group contains n = 4 spleens from 4 mice. Original magnification of images is 200 ×