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. 2020 Jul 16;26:71. doi: 10.1186/s10020-020-00195-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — In vitro treatment of ghrelin and GH have no effect on the immune response of splenocytes of aged septic rats. Rats were subjected to sham or CLP operation. Spleens were harvested at 20 h after CLP. Splenocytes were isolated and treated with ghrelin (Ghr), GH or GG in vitro, followed by the stimulation with LPS (100 ng/ml) for 5 h. The release of (a) TNF-α and (b) IL-6 in the medium were measured by ELISA. Data are expressed as mean ± SEM (n = 3 rats/group)