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. Author manuscript; available in PMC: 2020 Jul 16.
Published in final edited form as: Nat Protoc. 2019 Oct 30;14(12):3366–3394. doi: 10.1038/s41596-019-0223-x

Figure 4 |.

Figure 4 |

Design and operation of an 8-unit MOWChIP device. (a) Picture (left) and microscopic image (right) of an 8-unit MOWChIP device. (b) Loading immunomagnetic beads into the chamber with the assistance of a magnet. The sieve valves are open. (c) Flowing chromatin fragments through the packed bed of beads. The sieve valves are closed under 30 p.s.i. pressure. (d) Oscillatory washing under alternating pressure pulses applied via the inlet and outlet. Low salt and high salt washing buffers are used to remove nonspecifically bound chromatin. (e) Flushing out unbounded chromatin and debris while retaining the beads using the magnet.