. Author manuscript; available in PMC: 2020 Jul 16.

Published in final edited form as: Nat Protoc. 2019 Oct 30;14(12):3366–3394. doi: 10.1038/s41596-019-0223-x

Table 1.

Primers used in ChIP-qPCR for quality control. The designed primers were ordered from Integrated DNA Technologies, Coralville, IA, USA.

Species	Histone mark	Positive (P) or negative (N) locus	Locus name	Primer
Human	H3K4me3 (designed for GM12878 cells)	P	C9orf3 (F)	CCT CCT CAG TTC TCC CAG ACT
		P	C9orf3 (R)	AGC TGA GGT GGT AAG ATG TGA C
		N	N1 (F)	TCA TCT GCA AAT GGG GAC AA
		N	N1 (R)	AGG ACA CCC CCT CTC AAC AC
Mouse	H3K4me3	P	Gapdh-2	Active motif cat. no. 71018
	H3K4me3	N	Negative set 1	Active motif cat. no. 71011
	H3K27me3/H3K9me3	P	Hoxc10	Active motif cat. no. 71019
	H3K27me3/H3K9me3	N	Negative set 3	Active motif cat. no. 71013
	H3K36me3/H3K79me2	P	Actb-1	Active Motif cat. no. 71015
	H3K36me3/H3K79me2	N	Negative set 1	Active motif cat. no. 71011
	H3K27ac (designed for mouse brain)	P	Zranb3 (F)	GAA TGT ACC AGC GTC TCT TCT C
		P	Zranb3 (R)	TTT CTC TTT GGC AGC CTC TC
		N	N3 (F)	GCC CAT AAA GAA GGA GCT AGA G
		N	N3 (R)	GCA GCT AGA GAC AAG AGT TCT G